Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova DPBS, 500mL, DNase/RNase/Protease/Endotoxin Tested, Sterile. 8 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, RNase Free) - 250ml
Phosphate Buffered Saline PBS (10X RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959194 10X ADJ BUF 1.0M NACL .5M THCL
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
Biotang Inc Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Carbonate Buffered Saline 5X, pH 9.0. 125 mM Sodium Carbonate, 750 mM Sodium Chloride, pH 9.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-530.
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Quality Biological Inc DPBS without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Medchemexpress LLC Pbs/cds Qds | 5 MG
PbS/CdS QDs (PbS/CdSe QD) is a fluorescent probe that can be used for in vivo fluorescence imaging in the second near-infrared window. The 2nd-NIR fluorescence imaging at ca.1300 nm using the PbS/CdS QDs results in the highest signal to background ratio with a low blurring for in vivo imaging.
- Fluorescent probe for in vivo fluorescence imaging in the second near-infrared window
- Highest signal to background ratio with low blurring for in vivo imaging at ca.1300 nm
Boca Scientific Inc Antibody Stabilizer PBS Base, 125ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
AG Scientific Inc Hygromycin B in PBS, 10X2 ML
The concentration of Hygromycin B in PBS buffer is 50 mg/ml. Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.CAS Number: 31282-04-09Molecular Weight: 527.53Chemical Formula: C20H37N3O13Solubility: Soluble in WaterStorage Temperature: 2-8CResearch or further manufacturing use only, not for food or drug use.
Medchemexpress LLC PBS Buffer (10x) | 1 L
Phosphate-Buffered Saline (PBS) is a balanced salt solution primarily utilized for various cell culture applications, including washing cells, diluting cells, and preparing reagents. MedChemExpress's PBS Buffer (10x) is filtered, free of Mg2+, Ca2+, and phenol red, and has a pH ranging from 7.2 to 7.4.
- Filtered
- Free of Mg2+
- Free of Ca2+
- Free of phenol red
- pH is 7.2-7.4
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 50x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO