Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | 1629128-52-4 | MFCD18071452 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502156986.JPG-250.jpg)
eMolecules benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | 1629128-52-4 | MFCD18071452 | 1g
Pharmablock | benzyl exo-3-(hydroxymethyl)-8-azabicyclo[3.2.1]octane-8-carboxylate | 1g | 551164315 | PBV0006 | | 1629128-52-4 | MFCD18071452 | 275.348 | C16H21NO3
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Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration50KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration25KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Ethos Biosciences Buffer blocker phosphate casein 1/EA
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies enzymes or other proteins leading to clearer more reliable signals Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing Choose one of our other blocking buffers if your assay is detecting phosphoproteins uses biotin/streptavidin detection Alkaline Phosphatase detection autoimmune assays or aldehyde coated substrates in case of potential interference
Ethos Biosciences Buffer Diluent phosphate concentrate 1/EA
Dilurex 1X PBS is a ready-to-use working concentration of pH 7 4 phosphate buffered saline Skip the tedium of weighing powders and stocking raw materials that can expire Our PBS is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today
Ethos Biosciences Buffer blocker phosphate casein 1/EA
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies enzymes or other proteins leading to clearer more reliable signals Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing Choose one of our other blocking buffers if your assay is detecting phosphoproteins uses biotin/streptavidin detection Alkaline Phosphatase detection autoimmune assays or aldehyde coated substrates in case of potential interference
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 10G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 10G
![eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159302.JPG-250.jpg)
eMolecules tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 220598-43-6 | MFCD18073347 | 1g
Pharmablock | tert-butyl 4-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 1g | 551175136 | PBZ0195 | | 220598-43-6 | MFCD18073347 | 213.277 | C11H19NO3
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Rockland Immunochemicals 1.0 M Tris HCl pH 7.4 - MB-002
Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution (tris(hydroxymethyl)aminomethane hydrochloride) is useful for stabilization of buffer formulations and electrophoresis of biological molecules. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
eMolecules tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | 2167889-81-6 | MFCD30497054 | 1g
Pharmablock - Stock | tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | 1g | 521639997 | PBU1776 | | 2167889-81-6 | MFCD30497054 | 243.347 | C13H25NO3
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Teknova 1X PBS with 4mM EDTA, pH 7.4. 500mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.